Journal: Biosensors
Article Title: Exploring the Interaction of Biotinylated FcGamma RI and IgG1 Monoclonal Antibodies on Streptavidin-Coated Plasmonic Sensor Chips for Label-Free VEGF Detection
doi: 10.3390/bios14120634
Figure Lengend Snippet: A schematic of the VEGF SPR biosensor setup. Biotinylated FcγRI is immobilized on the streptavidin-coated sensor surface. The mAb is captured by the immobilized FcγRI through high-affinity interactions with the mAb Fc regions, orienting the mAb Fab regions for the optimal detection and quantification of VEGF. Created in BioRender. Khalid-Salako, F. (2024) https://BioRender.com/g42b753 .
Article Snippet: The Sensor chip SA with streptavidin pre-coated on a carboxymethylated dextran matrix surface functionalization was inserted and docked on the Biacore T200 instrument (Cytiva, Marlborough, MA, USA), which was then primed following the manufacturer’s instructions.
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